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Laboratory Technician II (S)
Category: Education
  • Your pay will be discussed at your interview

Job code: lhw-e0-90672095

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University of Rochester

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  Job posted:   Thu Jun 7, 2018
  Distance to work:   ? miles
  1 Views, 0 Applications  
Laboratory Technician II (S)
Full Time 40 hours Grade 075 Microbiology & Immunology M&D;
8:30 AM-5 PM
**General Purpose: **
The department of Microbiology and Immunology is seeking a Full-Time Lab Tech II to work in a basic science laboratory and conduct research relating to the vascular complications in HIV infected individuals. The work involves handling HIV infected blood samples.
**Typical Duties:**
This individual will process blood samples (HIV infected and uninfected) on a daily basis using the protocols already set up in the lab and keep the paperwork associated with it up to date. Initially, the candidate will conduct assigned experiments, working with daily supervision. It is expected that the individual will develop more independence over time in performing the experiments. It is very necessary to strictly adhere to the protocols to avoid manual error in sample processing.
Collect blood samples, isolate Peripheral Blood Mononuclear Cells (PBMCs) via density gradient centrifugation using Ficoll reagent, cryopreserve PBMCs. Maintain lab safety and cleanliness. Performs routine maintenance on equipment.
Fix blood samples using 4% Paraformaldehyde and process for cell staining using intracellular as well as cell surface markers of inflammation. Acquire the stained samples using flow cytometry.
Maintain the sample processing and storage log and analyze the flow cytometry data.
Isolate DNA and perform Polymerase Chain Reaction (PCR) for Telomere length. Run enzyme-linked immunosorbent assays (ELISA) for various plasma proteins.
Transfer samples to the central storage facility and attend monthly project related meetings. Perform general laboratory duties such as preparing reagents and restocking supplies.
* Associates degree in a related field; bachelor's degree preferred. Previous instruction in theoretical aspects of cell and molecular biology is highly desirable.
* Strong attention to detail and experience with basic Cell Culture techniques, DNA extraction and PCR.
* Excellent written and verbal communication and interpersonal skills.
* Previous experience with Flow Cytometry, handling blood samples and ELISA is highly desirable.
*EOE Minorities/Females/Protected Veterans/Disabled*
*Job Title:* Laboratory Technician II (S)
*Location:* School of Medicine & Dentistry
*Job ID:* 208871
*Regular/Temporary:* Regular
*Full/Part Time:* Full-Time

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